The BisulFlash™ DNA Modification Kit is a c o mplete set of optimized buffers and reagents to perform DNA modification using a next generation DNA bisulfite conversion technology developed by Epigentek. Through a proprietary c o mposition which allows DNA denaturation and bisulfite conversion to be processed at the same time, the c o mplete procedure is reduced to only 30 minutes. Furthermore, it prevents more than 90% of DNA loss, c o mpletely converting unmethylated cytosine into uracil.

Perfecting Bisulfite Conversion
Traditional methods involve a separate denaturation step followed by a subsequent sodium bisulfite DNA conversion step — but with the BisulFlash™ method, DNA denaturation status is concurrently sustained throughout the entire bisulfite DNA conversion process. This breakthrough approach enables the DNA conversion process to be significantly faster with higher conversion efficiency and ac c uracy. We continue to innovate with the development of the new BisulFlash™ kit by identifying four critical c o mponents of bisulfite conversion:

• Speed: Reduce the entire procedure to as short as 30 minutes without any reagent setup time.
• Efficiency: Completely convert unmethylated cytosine into uracil — modified DNA > 99.99%.
• DNA Protection: Protect against DNA degradation of which more than 90% of DNA loss can be prevented, allowing for greater recovery.
• Sensitivity: Start with the lowest amount of input DNA for modification — only 0.2 ng or just 50 cells.

The convenient ready-to-use, DNA conversion mix solution and single temperature incubation along with the features mentioned above allow for true perfection in bisulfite conversion. The BisulFlash™ DNA Modification Kit is suitable for MS-PCR, real time MS-PCR, methylation microarray, and pyrosequencing. Additionally, based on its ability for a c o mplete cytosine conversion, it is specifically suitable for next generation methylation sequencing/pyrosequencing.

Comparative Overview of Commercial Kits
Data was obtained through actual use of the kit, customer feedback, or i n f ormation provided by the supplier‘s datasheet or website.